Dnmt1 Rabbit Polyclonal Antibody

SKU: orb10546

Description

Dnmt1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: WB
• Dilution range: WB=1:500-2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Dnmt1 (21-120/1616aa)
• Target: DNMT1
• Molecular Weight: 178 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

AIM; CXXC finger protein 9; CXXC-type zinc finger protein 9; CXXC9; DNA(cytosine 5 ) methyltransferase 1; DNA(cytosine-5)-methyltransferase 1; DNA methyltransferase 1; DNA methyltransferase HsaI; DNA methyltransferase M.HsaI.; DNA MTase; DNA MTase HsaI; DNMT 1; DNMT; Dnmt1; Dnmt1; DNMT1_HUMAN; Dnmt1o; FLJ16293; HSN1E; M.HsaI; MCMT; Met1; MGC104992; mMmul; MommeD2.

Sample: Bone (Mouse) Lysate at 40 ug, Lymph node (Rat) Lysate at 40 ug, Primary: Anti-Dnmt1 (orb10546) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 178kD, Observed band size: 144 kD.

Quick Database Links

Gene Symbol

DNMT1

UniProt

P26358

References

Huang, Chao et al. Expression of DNA methyltransferases and target microRNAs in human tissue samples related to sporadic colorectal cancer Oncol Rep, 36, 2705-2714 (2016)

PubMed: 27666771