Dnmt1 Rabbit Polyclonal Antibody

• Catalog Number: orb10546
• Category: Antibodies
• Description: Dnmt1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Dnmt1 (21-120/1616aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 178 kDa
• Target: DNMT1
• UniProt ID: P26358
• RRID: AB_10751106
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AIM; CXXC finger protein 9; CXXC-type zinc finger
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Huang, Chao et al. Expression of DNA methyltransferases and target microRNAs in human tissue samples related to sporadic colorectal cancer Oncol Rep, 36, 2705-2714 (2016)

PubMed: 27666771

Sample: Bone (Mouse) Lysate at 40 ug, Lymph node (Rat) Lysate at 40 ug, Primary: Anti-Dnmt1 (orb10546) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 178kD, Observed band size: 144 kD.