DNASE2 Antibody

SKU: orb1239023

Description

DNASE2 Antibody

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: DNase II antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DNase II.The immunogen is located within the last 50 amino acids of DNase II.
• Target: DNASE2
• Molecular Weight: Aprox: 40 kDa
• Purification: DNase II Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: DNase II Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNase II Antibody: DNL, DNL2, DNASE2A, Deoxyribonuclease-2-alpha, Acid DNase, DNase II alpha

Western blot analysis of DNase II in human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with DNase antibody II at 1:500 dilution.

Immunohistochemistry of DNaseII in human spleen tissue with DNaseII antibody at 5 µg/mL.

Immunofluorescence of DNase II in human spleen tissue with DNase II antibody at 5 µg/ml. Green: DNase II antibody (orb1239023) Red: Phylloidin staining Blue: DAPI staining.

Immunohistochemistry of DNase II in human spleen tissue with DNase II antibody at 5 µg/ml.

Quick Database Links

Gene Symbol

DNASE2

UniProt

O00115

RefSeq:AF047016