DNASE2/DNase II Antibody

SKU: orb1538600

Description

DNASE2/DNase II Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution range: ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
• Reactivity: Human
• Application Notes:
  Further information: DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 ug/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Aprox: 40 kDa

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A 14 AA peptide near the C-Terminus of human DNase II. The immunogen is located within the last 50 AA of DNase II.
• Target: DNASE2 / DNase II
• Purification: Peptide Column Affinity Chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

DNASE2, Acid DNase, DNase II, DNase II alpha, DNASE2A, Deoxyribonuclease II alpha, Deoxyribonuclease-2-alpha, DNase II, lysosomal, R31240_2, DNL, DNL2, Lysosomal DNase II

Immunofluorescence of DNase II in human spleen tissue with DNase II antibody at 5 µg/ml. Green: DNase II antibody Red: Phylloidin staining Blue: DAPI staining

Quick Database Links

Gene Symbol

DNASE2 / DNase II