DNASE2/DNase II Antibody

• Catalog Number: orb1538600
• Category: Antibodies
• Description: DNASE2/DNase II Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: A 14 AA peptide near the C-Terminus of human DNase II. The immunogen is located within the last 50 AA of DNase II.
• Concentration: 1 mg/ml
• Dilution range: ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
• Conjugation: Unconjugated
• Target: DNASE2 / DNase II
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Alternative names: DNASE2, Acid DNase, DNase II, DNase II alpha, DNAS
• Note: For research use only
• Application notes: Further information: DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 ug/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Aprox: 40 kDa
• Expiration Date: 12 months from date of receipt.

Immunofluorescence of DNase II in human spleen tissue with DNase II antibody at 5 µg/ml. Green: DNase II antibody Red: Phylloidin staining Blue: DAPI staining