POLH Antibody

SKU: orb1258432

Description

POLH Antibody

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-320 of human DNA polymerase eta (NP_006493.1).
• Target: POLH
• Molecular Weight: Observed: 70kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RAD3RAD30A, XP-V, XPV, DNA polymerase eta, RAD30 homolog A, polymerase (DNA directed), eta, polymerase (DNA) eta, xeroderma pigmentosum variant type protein

Western blot analysis of extracts of various cell lines, using DNA polymerase eta antibody (orb1258432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of U2OS cells using DNA polymerase eta antibody (orb1258432). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using DNA polymerase eta antibody (orb1258432). Green：GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Quick Database Links

Gene Symbol

POLH

UniProt

Q9Y253