DNA PKcs (Phospho-Ser2056) Rabbit mAb [KD Validated]

SKU: orb1727814

Description

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution range: WB:1:500~1:2000IHC:1:50~1:200ICC/IF:1:50~1:200
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Phospho-DNA PKcs (S2056)
• Molecular Weight: 469kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Disclaimer: For research use only

Alternative Names

DNA- PKcs, DNA-dependent protein kinase catalytic subunit, DNPK1, EC 2.7.11.1, P460, PRKD, PRKDC, XRCC7, kinase DNA-PK

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human colon, using Phospho-DNA PKcs (S2056) Antibody.

Western blot analysis of Phospho-DNA PKcs (Ser2056) expression in alkaline treated Jurkat cell lysate.

Quick Database Links

UniProt

P78527