DMOG

SKU: orb1307042

Description

DMOG (Dimethyloxalylglycine) is a cell-permeable competitive antagonist of α-ketoglutarate that inhibits HIF prolyl hydroxylase (HIF-PH), leading to HIF-1α stabilization. This action induces autophagy and promotes angiogenesis, making it a valuable tool for studying hypoxia pathways, ischemic disease models, and angiogenesis in both cellular and animal research.

Research Area

Cell Biology, Neuroscience

Key Properties

• CAS Number: 89464-63-1
• MW: 175.14
• Purity: >98%
• Formula: C₆H₉NO₅
• SMILES: COC(=O)CNC(=O)C(=O)OC
• Target: Prolyl hydroxylase
• Solubility: Soluble in DMSO (up to 25 mg/ml) or in Ethanol (up to 25 mg/ml).

Bioactivity

• Target IC50:
  Hydroxyproline (chicken embryo lungs):9.3 μM|Cellular permeable prolyl-4-hydroxylase:5.18 μM|Hydroxyproline in chicken embryo lungs (medium):3.7 μM|JMJD2A:2.5 μM
• In Vivo:
  DMOG inhibits endogenous HIF inactivation and induces angiogenesis in the ischaemic skeletal muscles of mice. Up-regulation of hypoxia-inducible factor-1α by DMOG may be the cardioprotective mechanism of ischemic postconditioning in hyperlipidemic rats.
• In Vitro:
  DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC.
• Cell Research:
  To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells. (Only for Reference)

Storage & Handling

• Storage: -20°C / protect from light
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dimethyloxalylglycine

Compound Identifiers

PubChem CID

560326