DMD Antibody

• Catalog Number: orb1260091
• Category: Antibodies
• Description: DMD Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 346-635 of human DMD (NP_004007.1).
• Concentration: batch dependent
• Dilution range: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 427kDa
• Target: DMD
• UniProt ID: P11532
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: BMD, CMD3B, DXS142, DXS164, DXS206, DXS23DXS239, D
• Note: For research use only
• Application notes: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse heart, using DMD antibody (orb1260091) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat heart using DMD antibody (orb1260091) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using DMD antibody (orb1260091) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using DMD antibody (orb1260091) at dilution of 1:100. Blue: DAPI for nuclear staining.