DLL3 Antibody (C-term)

SKU: orb1927976

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:25, WB - 1:1000, IHC-P-Leica - 1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB23871
• Immunogen: This DLL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-548 amino acids from the C-terminal region of human DLL3. Antigen Region: 519-548 aa.
• Target: DLL3
• Molecular Weight: 64618 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Delta-like protein 3, Drosophila Delta homolog 3, Delta3, DLL3

All lanes: Anti-DLL3 Antibody (C-term) at 1:2000 dilution. Lane 1: A375 whole cell lysate. Lane 2: human brain lysate. Lane 3: human liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-DLL3 Antibody (C-term) at 1:1000 dilution. Lane 1: NCI-H1299 whole cell lysate. Lane 2: Human brain tissue lysate. Lane 3: Mouse brain tissue lysate. Lane 4: Rat brain whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human brain tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human testis tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-DLL3 Antibody (C-term) at 1:1000 dilution. Lane 1: A375 whole cell lysate. Lane 2: U-251 MG whole cell lysate. Lane 3: NCI-H1299 whole cell lysate. Lane 4: Human brain lysate. Lane 5: Mouse brain lysate. Lane 6: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling DLL3 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling DLL3 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

Quick Database Links

Gene Symbol

DLL3

UniProt

Q9NYJ7

RefSeq (Protein):NP_058637.1, NP_982353.1