DLL3 Antibody (C-term)

• Catalog Number: orb1937511
• Category: Antibodies
• Description: DLL3 Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9NYJ7
• MW: 64618 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: WB - 1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Research Area: Cell Biology, Neuroscience, Stem Cell & Developmen
• Note: For research use only

All lanes: Anti-DLL3 Antibody (C-term) at 1:1000 dilution. Lane 1: TT whole cell lysate. Lane 2: Human brain lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-DLL3 Antibody (C-term) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: Human brain lysate. Lane 4: SH-SY5Y whole cell lysate. Lane 5: U-2OS whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of DLL3 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

DLL3 Antibody (C-term) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the DLL3 antibody detected the DLL3 protein (arrow).

Immunohistochemical analysis of paraffin-embedded Human brain tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.