DKK1 Antibody

• Catalog Number: orb1258111
• Category: Antibodies
• Description: DKK1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 71-266 of human DKK1 (NP_036374.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 29kDa
• Target: DKK1
• UniProt ID: O94907
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: DKK1, DKK-1, SK
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse liver, using DKK1 antibody (orb1258111) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.