DKK1 Antibody

SKU: orb18888

Description

Goat polyclonal antibody to DKK1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 38kDa band observed in human spleen and bone marrow lysates after 1 μg/ml antibody staining. Please note that a band of similar size is reported in the literature (PMID 10383463). However, the calculated size is 28.7kDa according to NP_36374. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: DKK1
• Molecular Weight: 28.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 10 μg/ml
• Disclaimer: For research use only

Alternative Names

anti DKK1 antibody, anti SK antibody, anti DKK-1 antibody, anti dickkopf homolog 1 (Xenopus laevis) antibody, anti dickkopf-1 like antibody, anti dickkopf (Xenopus laevis) homolog 1 antibody, anti dickkopf homolog 1 antibody, anti dickkopf related protein-1 antibody

orb18888 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

orb18888 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb18888 (10µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb18888 (1µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Western blot analysis of Human Bone Marrow lysate using DKK1 antibody

Quick Database Links

Gene Symbol

DKK1

NCBI Gene ID

22943