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Description
Research Area
Metabolism Research
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This DIO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human DIO3. |
| Target | DIO3 |
| Molecular Weight | 34 kDa |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Type III iodothyronine deiodinase, 5DIII, DIOIII, Type 3 DI, Type-III 5'-deiodinase, DIO3, ITDI3, TXDI3
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Western blot analysis of DIO3 Antibody in HepG2 cell line lysates (35 ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DIO3 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
DIO3 Antibody (orb1262173)
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