DIO3 Antibody (C-term)

SKU: orb40229

Description

Peptide affinity purified rabbit polyclonal antibody.

Research Area

Cancer Biology, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:50-100
• Reactivity: Human, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD46201
• Immunogen: Synthetic Peptide
• Target: DIO3
• Molecular Weight: 33947
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-5DIII antibody, anti-DIOIII antibody, anti-Type 3 DI antibody, anti-Type-III 5'-deiodinase antibody, anti-Type III iodothyronine deiodinase antibody, anti-TXDI3 antibody, anti-ITDI3 antibody, anti-DIO3 antibody

Anti-DIO3 Antibody (C-term) at 1:2000 dilution + Human placenta lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-DIO3 Antibody (C-term) at 1:2000 dilution + HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-DIO3 Antibody (C-term) at 1:2000 dilution + Rat skeletal muscle tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DIO3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

DIO3

UniProt

P55073

RefSeq (Protein):NP_001353.4