DHRS2 Antibody

• Catalog Number: orb1257004
• Category: Antibodies
• Description: DHRS2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DHRS2 (NP_878912.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 30-35kDa
• Target: DHRS2
• UniProt ID: Q13268
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: DHRS2, HEP27, SDR25C1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using DHRS2 antibody (orb1257004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human breast cancer using DHRS2 antibody (orb1257004) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterine cancer using DHRS2 antibody (orb1257004) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using DHRS2 antibody (orb1257004) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using DHRS2 antibody (orb1257004). Blue: DAPI for nuclear staining.