DHRS2 Rabbit Polyclonal Antibody

• Catalog Number: orb221392
• Category: Antibodies
• Description: DHRS2 Rabbit Polyclonal Antibody
• Target: DHRS2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human DHRS2 (61-160/258aa)
• UniProt ID: Q13268
• MW: 27 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Dehydrogenase/reductase(SDR family) member 2; Dehy
• Note: For research use only

Protein: Hela (human) lysates at 40 ug, Primary: rabbit Anti-DARC (orb221392) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 27 kD, Observed band size: 30 kD.

Protein: liver (mouse) lysates at 30 ug, Primary: Anti-DHRS2 (orb221392) at 1:300, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:3000, ECL excitated the fluorescence, Predicted band size: 27 kD, Observed band size: 27/75 kD.

Protein: lung (mouse) lysates at 40 ug, Primary: Anti-DHRS2 (orb221392) at 1:300, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000, ECL excitated the fluorescence, Predicted band size: 27 kD, Observed band size: 27 kD.

Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated (orb221392) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DHRS Polyclonal Antibody, Unconjugated (orb221392) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.