DEPDC1B/BRCC3 Polyclonal Antibody

SKU: orb1420133

Description

Rabbit polyclonal antibody to DEPDC1B.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Disclaimer: For research use only

Alternative Names

DEP domain-containing protein 1B, HBV X-transactivated gene 8 protein, HBV XAg-transactivated protein 8, DEPDC1B, XTP8

Sample: HepG2 Cell (Human) Lysate at 30 ug. A431 Cell (Human) Lysate at 30 ug. Primary: Anti-DEPDC1B/BRCC3 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 62kD. Observed band size: 62kD.

Tissue/cell: human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-BRCC3 Polyclonal Antibody, Unconjugated 1: 500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

UniProt

Q8WUY9