DEPDC1B/BRCC3 Polyclonal Antibody

• Catalog Number: orb1420133
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DEPDC1B.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Rat
• Conjugation: Unconjugated
• UniProt ID: Q8WUY9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: DEP domain-containing protein 1B, HBV X-transactiv
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: HepG2 Cell (Human) Lysate at 30 ug. A431 Cell (Human) Lysate at 30 ug. Primary: Anti-DEPDC1B/BRCC3 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 62kD. Observed band size: 62kD.

Tissue/cell: human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-BRCC3 Polyclonal Antibody, Unconjugated 1: 500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.