DEPDC1B/BRCC3 Rabbit Polyclonal Antibody

SKU: orb183064

Featured

Description

Research Area

Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human
Predicted Reactivity	Bovine, Mouse, Rabbit, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human DEPDC1B/BRCC3 (451-529/529aa)
Target	DEPDC1B
Molecular Weight	62 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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BRCC3; Breast cancer cell 3; DEP domain containing 1B; DEP domain containing protein 1B; FLJ11252; DEP1B_HUMAN; HBV X transactivated gene 8 protein; HBV X-transactivated gene 8 protein; HBV XAg-transactivated protein 8; HBxAg transactivated protein 1; OTTHUMP00000122605; OTTHUMP00000221973; XTP1; XTP8.

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DEPDC1B/BRCC3 Rabbit Polyclonal Antibody

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human MCF-7 cell lysates, Primary: Anti-DEPDC1B/BRCC3 (orb183064) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kDa, Observed band size: 68 kDa.

Tissue/Cell: human brain glioma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-BRCC3 Polyclonal Antibody, Unconjugated (orb183064) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.