DEPDC1B/BRCC3 Rabbit Polyclonal Antibody

• Catalog Number: orb183064
• Category: Antibodies
• Description: DEPDC1B/BRCC3 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Mouse, Rabbit, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DEPDC1B/BRCC3 (451-529/529aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 62 kDa
• Target: DEPDC1B
• UniProt ID: Q8WUY9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: BRCC3; Breast cancer cell 3; DEP domain containing
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Posor, York et al. Local synthesis of the phosphatidylinositol-3,4-bisphosphate lipid drives focal adhesion turnover Dev Cell, (2022)

PubMed: 35809565

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human MCF-7 cell lysates, Primary: Anti-DEPDC1B/BRCC3 (orb183064) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kDa, Observed band size: 68 kDa.

Tissue/Cell: human brain glioma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-BRCC3 Polyclonal Antibody, Unconjugated (orb183064) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.