DENND1B Antibody

SKU: orb1270847

Description

DENND1B Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  For WB starting dilution is: 1:500For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This DENND1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the N-terminal region of human DENND1B.
• Target: DENND1B
• Molecular Weight: 48 kDa
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

DENN domain-containing protein 1B, Connecdenn 2, Protein FAM31B, DENND1B, C1orf218, FAM31B

Western Blot at 1:500 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in NCI-H460 cell line lysates (35 ug/lane).

DENND1B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Quick Database Links

Gene Symbol

DENND1B

UniProt

Q6P3S1

RefSeq:Q6P3S1