DENND1B Antibody

SKU: orb1880976

Description

The DENND1B Antibody is suitable for FC, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human DENND1B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200), FC (1/10 - 1/50)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human DENND1B. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

C1orf218; FAM31B; DENN domain-containing protein 1B; Connecdenn 2; Protein FAM31B

Western blot analysis of DENND1B expression in MOLT4 (A) whole cell lysates. (Predicted band size: 86 kD; Observed band size: 48 kD)

Immunohistochemical analysis of DENND1B staining in human lymph formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of NCIH460 cells using Anti-DENND1B Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Quick Database Links

UniProt

Q6P3S1

NCBI Gene ID

163486