DEFA1/3 Antibody

• Catalog Number: orb1249192
• Category: Antibodies
• Description: DEFA1/3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DESLAPKHPGSRKN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 10kDa band observed in Human Spleen lysates (calculated MW of 10.2kDa according to NP_004075.1). . An additional band of unknown identity was also consistently observed at 20kDa, and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. . Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 3-6ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DEFA1/3
• UniProt ID: P59665
• NCBI: NP_004075.1, NP_005208.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: DEFA1, defensin, alpha 1, myeloid-related sequence
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 10kDa band observed in Human Spleen lysates (calculated MW of 10.2kDa according to NP_004075.1). . An additional band of unknown identity was also consistently observed at 20kDa, and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. . Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 3-6ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249192 (2 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer).Detected by chemiluminescence.

orb1249192 (3.8 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.