Goat anti-DEFA1/3 precursor Antibody

• Catalog Number: orb20493
• Category: Antibodies
• Description: Goat polyclonal antibody to alpha Defensin 1
• Target: DEFA1/3 precursor
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DESLAPKHPGSRKN
• RRID: AB_10752662
• MW: 10.2
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 3-6 μg/ml
• Application notes: This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 10kDa band observed in Human Spleen lysates (calculated MW of 10.2kDa according to NP_004075.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DEF1 antibody, anti DEFA1 antibody, anti DEFA
• Note: For research use only
• Entrez: 1668, 1667

Western blot analysis of Human Spleen lysate using alpha Defensin 1 antibody

Immunohistochemical staining of Human Thymus using alpha Defensin 1 antibody