Decorin Antibody

• Catalog Number: orb1716278
• Category: Antibodies
• Description: Decorin is a small leucine-rich proteoglycan (SLRP) family member that consists of a glycosaminoglycan chain-containing core protein. The core protein contains ten leucine rich repeats that contain sites for glycosylation, flanked by disulfide bond stabilizing loops. Decorin binds to Collagen Type I, II and IV in vivo and promotes the formation of fibers with variations in stability and solubility. The Decorin core protein binds to growth factors, intercellular matrix molecules, such as Fibronectin and Thrombospondin, and to the Decorin endocytosis receptor. Decorin binds to and inhibits TGF may contribute to cartilage destruction that is characteristic of inflammatory joint diseases. For immunostaining, pre-incubation with chondroitinase-SBC or testicular hyaluronidase may be required to expose the epitope.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: DCN/6289
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, lambda
• Immunogen: Recombinant full-length protein was used as the immunogen for the Decorin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Decorin antibody is available for research use only.
• UniProt ID: P07585
• Storage: Aliquot the Decorin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the Decorin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human placental tissue with Decorin antibody (clone DCN/6289) at 2 ug/ml. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding.

IHC staining of FFPE human prostate tissue with Decorin antibody (clone DCN/6289). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.