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Catalog Number | orb1929956 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB19595 |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Porcine, Sheep |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 39747 Da |
Target | This DCN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-150 amino acids from the Central region of human DCN. |
UniProt ID | P07585 |
NCBI | NP_598014.1, NP_598011.1, NP_598013.1, NP_598010.1, NP_598012.1, NP_001911.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Decorin, Bone proteoglycan II, PG-S2, PG40, DCN, S Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
DCN Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with DCN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of DCN antibody (Center) in mouse heart tissue lysates (35 ug/lane). DCN (arrow) was detected using the purified Pab.
Western blot analysis of DCN (arrow) using rabbit polyclonal DCN Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DCN gene.