DDX6 Antibody

• Catalog Number: orb1259076
• Category: Antibodies
• Description: DDX6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human DDX6 (NP_001244120.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 54kDa
• Target: DDX6
• UniProt ID: P26196
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: DDX6, P54, RCK, HLR2
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using DDX6 antibody (orb1259076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat brain using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human mammary cancer using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of L929 cells using DDX6 Polyclonal Antibody (orb1259076) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.