DEAD-box protein 6 Antibody

• Catalog Number: orb19176
• Category: Antibodies
• Description: Goat polyclonal antibody to DDX6
• Target: DEAD-box protein 6
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: AEYHSEPVEDEKP
• RRID: AB_10747138
• MW: 53.2
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 50kDa band observed in lysates of cell line Jurkat (calculated MW of 53.2kDa according to NP_NP_004388.1). In transfected HEK293 transiently expressing Human DDX6 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. The endogenous 53kDa protein is observed in both. Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DEAD-box protien 6 antibody, anti DEAD (Asp-G
• Research Area: Cancer, Epigenetics
• Note: For research use only
• Entrez: 1656

Western blot analysis of staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35µg protein in RIPA buffer) using DDX6 antibody.

Immunohistochemical staining of Human Cortex using DDX6 antibody

Immunohistochemical staining of Human Thymus using DDX6 antibody