DDX4 Antibody

SKU: orb248168

Description

DEAD box proteins are RNA helicases involved in cellular processes involving the alteration of RNA secondary structure. DDX4, DEAD box polypeptide 4, is the mammalian homolog of the Drosophila VASA gene. DDX4 is expressed solely in ovarian and testicular germ cells and therefore a useful germ cell lineage marker and possible useful biomarker for germ cell cancers.

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot: 2-5ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from the C-terminal region of DDX4/VASA was used as the immunogen for the DDX4 antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.5 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human samples with DDX4 antibody at 2 ug/ml.