DDX4 antibody

• Catalog Number: orb5967
• Category: Antibodies
• Description: DDX4 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Bovine, Canine, Gallus, Human, Mouse, Porcine, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DDX4. Please contact us for the exact immunogen sequence. The peptide is available as orb374780.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: IHC-P: 1:100-800, WB: 1:100-800
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 80 kDa
• Target: DDX4
• Entrez: 54514
• UniProt ID: Q61496, Q9NQI0, Q64060
• NCBI: 11, 077726, 024415, 21
• RRID: AB_10923699
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-MVH antibody, anti-MVH antibody, anti-DDX4/MV
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Ying-Jie Niu 1, Wenjie Ren 2, Guangzheng Liu 2, Kai Jin 3, Dan Zheng 2, Qisheng Zuo 2, Yani Zhang 2, Xiang-Shun Cui 4, Guohong Chen 2, Bichun Li 5 Clonally derived chicken primordial germ cell lines maintain biological characteristics and proliferative potential in long-term culture Theriogenology, 215, 67-77 (2024)

PubMed: 38011785

Applications

WB

Reactivity

Gallus

Western blot analysis of rat brain (lane 1), rat large intestines (lane 2), mouse testis (lane 3); hela cells (lane 4) using DDX4 antibody (1 ug/ml)

Immunohistochemical staining of paraffin embedded mouse testis tissue using anti-DDX4 (primary antibody at 2.5 ug/ml)

IHC-P staining of pig small intestines tissue using DDX4 antibody (dilution at 2.5 ug/ml)

WB analysis of rat brain (lane 1), rat large intestines (lane 2), mouse testis (lane 3); hela cells (lane 4) DDX4 antibody (1 ug/ml)

Immunohistochemical staining of pig small intestines tissue using DDX4 antibody (dilution of primary antibody - 2.5 ug/ml)

Immunohistochemical staining of paraffin embedded pig small intestines tissue using DDX4 antibody (primary antibody at 2.5 ug/ml)

IHC-P image of mouse brain tissue using anti-DDX4 (dilution of primary antibody at 2.5 ug/ml)