DDX3Y Antibody

• Catalog Number: orb1261173
• Category: Antibodies
• Description: DDX3Y Antibody
• Target: DDX3Y
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_001116137.1).
• UniProt ID: O15523
• MW: Observed: 73kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ATP-dependent RNA helicase DDX3Y, DEAD box protein
• Research Area: Cell Biology
• Note: For research use only

Western blot analysis of extracts of various cell lines, using DDX3Y antibody (orb1261173) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat testis using DDX3Y antibody (orb1261173) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using DDX3Y antibody (orb1261173) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using DDX3Y antibody (orb1261173) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using DDX3Y antibody (orb1261173) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using DDX3Y Polyclonal Antibody (orb1261173) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using DDX3Y Polyclonal Antibody (orb1261173) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.