DDX1 Antibody

• Catalog Number: orb1256922
• Category: Antibodies
• Description: DDX1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human DDX1 (NP_004930.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 100kDa
• Target: DDX1
• UniProt ID: Q92499
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: DDX1, DBP-RB, UKVH5d
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using DDX1 antibody (orb1256922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat brain using DDX1 Antibody (orb1256922) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using DDX1 Antibody (orb1256922) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using DDX1 Antibody (orb1256922) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using DDX1 Antibody (orb1256922) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using DDX1 antibody (orb1256922). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 100 ug extracts of 293T cells using 3 ug DDX1 antibody (orb1256922). Western blot was performed from the immunoprecipitate using DDX1 antibody (orb1256922) at a dilution of 1:1000.