DDX1 Antibody

• Catalog Number: orb379640
• Category: Antibodies
• Description: Mouse monoclonal antibody to DDX1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3E5E2
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human DDX1 (AA: 642-740) expressed in E. Coli.
• Antibody Type: Primary Antibody
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 82.4kDa
• Target: DDX1
• Entrez: 1653
• UniProt ID: Q92499
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: DBP-RB; UKVH5d
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of DDX1 antibody

Flow cytometric analysis of Hela cells using DDX1 antibody

Immunofluorescence analysis of Hela cells using DDX1 antibody

Immunohistochemical staining of human bladder cancer tissue using DDX1 antibody

Immunohistochemical staining of human colon cancer tissue using DDX1 antibody

Western blot analysis of Hela (Lane 1), MCF-7 (Lane 2), A431 (Lane 3), PC-3 (Lane 4), NIH/3T3 (Lane 5), Jurkat (Lane 6), U251 (Lane 7), and HEK293 (Lane 8) cell lysate using DDX1 antibody

Western blot analysis of HEK293 cell lysate using DDX1 antibody

Western blot analysis of recombinant protein using DDX1 antibody