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Catalog Number | orb1258019 |
---|---|
Category | Antibodies |
Description | DDB1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide of human DDB1 |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 127kDa |
Target | DDB1 |
UniProt ID | Q16531 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | DDB1, damage-specific DNA binding protein 1, 127 k Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using DDB1 Antibody (orb1258019) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using DDB1 Antibody (orb1258019) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DDB1 Antibody (orb1258019) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DDB1 Antibody (orb1258019) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DDB1 Polyclonal Antibody (orb1258019) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DDB1 Polyclonal Antibody (orb1258019) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Gallus | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |