DDB1 Antibody

SKU: orb1258019

Description

DDB1 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide of human DDB1
• Target: DDB1
• Molecular Weight: Observed: 127kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

DDB1, damage-specific DNA binding protein 1, 127 kDa, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1, DDB p127 subunit, damage-specific DNA binding protein 1 (127kD)

Western blot analysis of extracts of various cell lines, using DDB1 Antibody (orb1258019) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat testis using DDB1 Antibody (orb1258019) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using DDB1 Antibody (orb1258019) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using DDB1 Antibody (orb1258019) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using DDB1 Polyclonal Antibody (orb1258019) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using DDB1 Polyclonal Antibody (orb1258019) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

DDB1

UniProt

Q16531