DDAH2 Antibody

SKU: orb1249424

Description

DDAH2 Antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in Human Lung lysates (calculated MW of 29.6kDa according to NP_039268.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunofluorescence:Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10ug/ml.Flow Cytometry:Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: SSLCLVLSTRPHS
• Target: DDAH2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

DDAH2, dimethylarginine dimethylaminohydrolase 2, DDAH, G6a, NG30, DDAHII, dimethylarginine dimethylaminohydrolase II, NG-dimethylarginine dimethylamino hydrolase homolog, OTTHUMP00000062666, DADB-110M10.5, OTTHUMP00000029307, OTTHUMP00000174406, alternative name: NG30, G6a, dimethylarginine dimethylaminohydrolase II

orb1249424 (0.1 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

DDAH2

UniProt

O95865

RefSeq (Protein):NP_039268.1