DAPK2 Antibody (N-term)

• Catalog Number: orb1929624
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This DAPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DAPK2.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q9UIK4
• MW: 42898 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:2000, IHC-P: 1:50~100
• Clone Number: RB3053
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Death-associated protein kinase 2, DAP kinase 2, D
• Note: For research use only
• NCBI: NP_055141.2

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Anti-DAPK2 Antibody (M1) at 1:2000 dilution + Hela whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from SH-SY5Y cell line and mouse brain tissue lysates (from left to right), using DAPK2 Antibody (M1). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.