CD55 Antibody

SKU: orb1239032

Description

CD55 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  DAF body can be used for detection of DAF by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: DAF antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF.The immunogen is located within amino acids 360 - 410 of DAF.
• Target: CD55
• Molecular Weight: Predicted: 48 kDa Observed: 49 kDa
• Purification: DAF antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: DAF antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Decay-accelerating factor, CD55 molecule decay accelerating factor for complement (Cromer blood group), CD55, CR, TC, CROM

Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 µg/ml.

Immunohistochemistry of DAF in human lung tissue with DAF antibody at 5 µg/ml.

Immunofluorescence of DAF in human lung tissue with DAF antibody at 20 µg/ml.

Western blot analysis of DAF1 expression in HeLa cell lysate with DAF1 antibody at (A) 1 and (B) 2 µg/ml.

Quick Database Links

Gene Symbol

CD55

UniProt

P08174

RefSeq (Protein):NP_001108224