Anti-CD55 [LU30]

SKU: orb411574

Description

Human monoclonal antibody to CD55

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: Human IgG
• Clone No.: LU30
• Immunogen: Human CD55.
• Target: CD55
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

DAF; Complement decay-accelerating factor

Flow-cytometry using the anti-CD55 (Complement decay-accelerating factor) antibody LU-30 U93 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of LU-30 (orb411574, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunohistochemical staining of human lung tissue using anti-CD55 antibody LU30 Anti-CD55 (Complement decay-accelerating factor)staining of paraffin embedded human lung tissue using the rabbit-chimeric version of LU30 (orb411574). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb411574 was carried out at 4 µg/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3, 3′-diaminobenzidine), and counter staining with haematoxylin. Strong staining of the epithelial surface of alveoli may be observed. Recommended concentration, 2-4 µg/ml.

Quick Database Links

Gene Symbol

CD55

UniProt

P08174

References

Ridgway, J. B. et al. Identification of a human anti-CD55 single-chain Fv by subtractive panning of a phage library using tumor and nontumor cell lines Cancer Res, 59, 2718-2723 (1999)

PubMed: 10363997