Multi Cytokeratin 4/5/6/8/10/13/18 Antibody [C11]

• Catalog Number: orb1252667
• Category: Antibodies
• Description: Multi Cytokeratin 4/5/6/8/10/13/18 Antibody [C11]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C11
• Tested applications: FC, IF, IHC, WB
• Reactivity: Bovine, Frog, Goat, Guinea pig, Human, Marmoset, Mouse, Porcine, Rat
• Isotype: IgG1
• Immunogen: A keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: use the Multi Cytokeratin mAb at 0.5-1 ug/million cellsIF: 0.5-1 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Flow Cytometry: use the Multi Cytokeratin mAb at 0.5-1 ug/million cellsIF: 0.5-1 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human colon carcinoma and Multi Cytokeratin antibody (clone C11).