Cytokeratin 8, CK8

• Catalog Number: orb1294306
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cytokeratin 8
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Dilution range: Western Blot 1:2000–1:3000 Immunofluorescence 1:500–1:800 Immunohistochemistry (Paraffin) 1:400–1:600
• Conjugation: Unconjugated
• Target: Cytokeratin 8, CK8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Cytokeratin 8 antibody - at 1/2000 dilution, Lysates at 35 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with orb1294306 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 8 with orb1294306 at 1/400.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 8 with orb1294306 at 1/400.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294306 Anti-Cytokeratin 8 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.