Cytokeratin 8 rabbit pAb

• Catalog Number: orb765010
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cytokeratin 8
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Keratin 8. AA range:401-450
• MW: 53
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-KRT8 antibody, anti-CYK8 antibody, anti-Kerat
• Note: For research use only

Western Blot analysis of various cells using Cytokeratin 8 Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of 293 cells using Cytokeratin 8 Polyclonal Antibody diluted at 1:2000.

Immunofluorescence analysis of HeLa cells, using Keratin 8 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Keratin 8 Antibody. The picture on the right is blocked with the synthesized peptide.