Cytokeratin 14 Antibody

SKU: orb248446

Description

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). Cytokeratin 14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. antibody to cytokeratin 14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. cytokeratin 14 antibody is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Cytokeratin 14 antibody is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-3ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG3, kappa
• Clone No.: LL002
• Immunogen: A synthetic peptide of 15 amino acid from the C-terminus of human Cytokeratin 14 was used as the immunogen.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human prostate with Cytokeratin 14 antibody

IHC testing of human prostate (10X) stained with Cytokeratin 14 antibody (LL002).

IHC testing of human prostate (20X) stained with Cytokeratin 14 antibody (LL002).

IHC testing of FFPE human prostate with Cytokeratin 14 antibody

Western blot testing of A431 cell lysate (epidermoid carcinoma) and Cytokeratin 14 antibody (clone LL002) at 1 ug/ml. Predicted/observed molecular weight: ~53kDa.