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Cytokeratin 18 Antibody

Catalog Number: orb749637

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb749637
CategoryAntibodies
DescriptionThis mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Species/HostMouse
ClonalityMonoclonal
Clone NumberKRT18/834
Tested applicationsFACS, IF, IHC-P, WB
ReactivityHuman, Rat
IsotypeMouse IgG1, kappa
ImmunogenRecombinant full-length human KRT18 protein was used as the immunogen for the Cytokeratin 18 antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Cytokeratin 18 antibody is available for research use only.
UniProt IDP05783
StorageStore the Cytokeratin 18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the Cytokeratin 18 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
Cytokeratin 18 Antibody

IHC: Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin 18 antibody (KRT18/834).

Cytokeratin 18 Antibody

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 18 antibody (KRT18/834).

Cytokeratin 18 Antibody

IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cytokeratin 18 antibody (KRT18/834).

Cytokeratin 18 Antibody

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 18 antibody (KRT18/834).

Cytokeratin 18 Antibody

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 18 antibody (KRT18/834).

Cytokeratin 18 Antibody

IHC: Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Cytokeratin 18 antibody (KRT18/834).

Cytokeratin 18 Antibody

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with Cytokeratin 18 antibody (KRT18/834).

Cytokeratin 18 Antibody

Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 18 antibody (clone KRT18/834, green) and Reddot nuclear stain (red).

Cytokeratin 18 Antibody

Flow cytometry testing of PFA-fixed human HeLa cells with Cytokeratin 18 antibody (clone KRT18/834); Red = isotype control, Blue = Cytokeratin 18 antibody.

Cytokeratin 18 Antibody

Western blot testing of human stomach, intestine and HCT-116 cell lysate with Cytokeratin 18 antibody (clone KRT18/834). Predicted molecular weight ~48 kDa.

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