Cytokeratin 10 + 13 Antibody [SPM262]

• Catalog Number: orb1252416
• Category: Antibodies
• Description: Cytokeratin 10 + 13 Antibody [SPM262]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM262
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Feline, Human
• Isotype: IgG2a, kappa
• Immunogen: A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10 + 13 antibody.
• Antibody Type: Primary Antibody
• Concentration: 0.2 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: KRT10, KRT13
• UniProt ID: P13645, P13646
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.25-0.5 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Cytokeratin 10 + 13 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human tonsil stained with Cytokeratin 10 + 13 antibody (SPM262).

Western blot testing of A431 cell lysate and Cytokeratin 10 + 13 antibody (clone SPM262).