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cytochrome P450 1A1 Antibody

SKU: orb1248146

Description

cytochrome P450 1A1 Antibody

Research Area

Metabolism Research

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 85-90kDa band observed in lysates of cell lines A549 and MCF7 (calculated MW of 58.2kDa according to NP_000490.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: CQEKQLDENANVQ
Targetcytochrome P450 1A1
Molecular WeightApprox 85-90kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CYP1A1, cytochrome P450, family 1, subfamily A, polypeptide 1, AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX, CYPIA1, OTTHUMP00000183469, aryl hydrocarbon hydroxylase, cytochrome P1-450, dioxin-inducible, cytochrome P450 1A1, cytochrome P450 form 6, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1, cytochrome P450-C, cytochrome P450-P1, flavoprotein-linked monooxygenase, xenobiotic monooxygenase

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Quality Guarantee

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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