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cytochrome P450 1A1 (mouse) Antibody

SKU: orb125228

Description

Goat Polyclonal to cyp1a1. NP_001129531.1, PMID: 21357676.

Research Area

Metabolism Research

Images & Validation

Tested ApplicationsELISA, IF, IHC, WB
Dilution RangeELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 10 μg/ml
ReactivityHuman, Mouse, Rat
Application Notes
WB: Approx 55kDa band observed in Human and Rat Lung lysates (calculated MW of 58.2kDa according to Human NP_000490.1 and 59.4kDa according to Rat NP_036672.). Primary incubation was 1 hour. Recommended concentration: 0.1-0.3µg/ml.. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 10µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of NIH3T3 cells. Recommended concentration: 10µg/ml.

Key Properties

ClonalityPolyclonal
Targetcytochrome P450 1A1 (mouse)
Protein SequenceQDRKLDENANVQLSD
Molecular Weight59.2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti cyp1a1 antibody, anti cytochrome P450, family 1, subfamily A, polypeptide 1 antibody, anti AHH antibody, anti AHRR antibody, anti CP11 antibody, anti Cyp1a2 antibody, anti P450-1 antibody, anti CYPIA1 antibody, anti aromatic compound inducible antibody, anti cytochrome P450 1A1 antibody, anti cytochrome P450 family 1 subfamily a polypeptide 2 antibody, anti cytochrome P450 subfamily I, polypeptide 1 antibody, anti cytochrome P450, 1a1, aromatic compound inducible antibody, anti cytochrome P450-P1 antibody

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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