Cytarabine hydrochloride

SKU: orb1708669

Description

Cytarabine hydrochloride

Research Area

Cell Biology, Infectious Disease & Virology, Molecular Biology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 69-74-9
• MW: 279.68
• Purity: 99.62% (May vary between batches)
• Formula: C₉H₁₄ClN₃O₅
• SMILES: O[C@@H]1[C@@H](O[C@H](CO)[C@H]1O)N2C(=O)N=C(N)C=C2.Cl
• Target: DNA/RNA Synthesis,HSV,Nucleoside Antimetabolite/Analog,Autophagy
• Solubility: DMSO:50 mg/mL (178.78 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.15 mM)

Bioactivity

• In Vivo:
  Cytarabine (250 mg/kg) induces placental growth retardation and elevates apoptosis of trophoblastic cells in the placental labyrinth zone in pregnant Slc:Wistar rats. This effect begins 3 hours post-treatment, peaks at 6 hours, and reverts to baseline at 48 hours. It notably increases p53 protein levels and the expression of p53 transcriptional target genes, such as p21, cyclinG1, fas, and activates caspase-3 . Additionally, Cytarabine effectively combats acute leukemias by causing a characteristic G1/S phase block and cell synchronization, thereby extending the survival of leukemic Brown Norway rats. Its effectiveness shows a weak dose-related pattern, suggesting that higher doses do not enhance its anti-leukemic effect in humans .
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• In Vitro:
  Cytarabine is converted into its active form, Ara-CTP (tri-phosphate), by the enzyme deoxycytidine kinase (dCK). It then competes with the natural nucleotide dCTP for incorporation into DNA, ultimately inhibiting DNA synthesis by disrupting DNA and RNA polymerases' functions. This compound exhibits potent growth inhibitory effects on wild-type CCRF-CEM cells, with an IC50 of 16 nM, showcasing higher efficacy compared to other acute myelogenous leukemia (AML) cells. Moreover, Cytarabine is capable of inducing apoptosis in rat sympathetic neurons at a concentration of 10 μM, with the highest observed toxicity at 100 μM, resulting in over 80% cell death within 84 hours. This process involves the release of mitochondrial cytochrome-c and activation of caspase-3, with noted attenuation of toxicity via p53 knockdown and a delay through bax deletion.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA polymerase, DNA Synthesis, DNASynthesis, Cytosine Arabinoside Hydrochloride, Cytosine Arabinoside hydrochloride, Cytarabine Hydrochloride, Ara-C hydrochloride, Ara-C Hydrochloride, RNASynthesis, RNA Synthesis

Compound Identifiers

PubChem CID

6252