CYLD Antibody

• Catalog Number: orb248160
• Category: Antibodies
• Description: CYLD is involved in multiple physiological processes including immune response, osteoclastogenesis and spermatogenesis. It is a tumor suppressor via its negative regulation of NF-kB activation. CYLD is a deubiquitinase, removing ubiquitin chains from tumor-necrosis factor receptor-associated factor 2 (TRAF2) and thereby preventing its activation of IkB, a regulator of NF-kB. NF-kB remains sequestered in the cytoplasm, unable to translocate to the nucleus and initiate cell survival and oncogenetic signaling.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from the N-termiinal region of human CYLD was used as the immunogen for this antibody.
• Dilution range: Western blot: 2-5ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CYLD antibody is available for research use only.
• Entrez: 1540
• Storage: Aliquot the CYLD antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Note: For research use only
• Application notes: Provided assay concentrations are suggestions only, CYLD antibody titration may be required for optimal results.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human samples using CYLD antibody (primary antibody dilution at: at 2 ug/ml)