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Item 1 of 2
| Catalog Number | orb19453 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to Cyld |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Mouse, Rat |
| Dilution range | ELISA: 1:32000, WB: 0.5-1.5 μg/ml, IHC-P: 5 μg/ml |
| Conjugation | Unconjugated |
| MW | 107 |
| Target | Cyld (mouse) |
| Protein Sequence | DSQQSKSKNPWYIDE |
| RRID | AB_10767387 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti CYLD antibody, anti cylindromatosis (turban t Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 125kDa band observed in mouse and rat brain lysates (calculated MW of 107kDa according to mouse NP_775545.1). Recommended concentration: 0.5-1.5 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Yang, Xifeng et al. Downregulation of miR‑181b inhibits human colon cancer cell proliferation by targeting CYLD and inhibiting the NF‑κB signaling pathway Int J Mol Med, 46, 1755-1764 (2020)
orb19453 (0.5ug/ml) staining of rat brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of Mouse Brain using Cyld antibody
