Goat anti-Cyld (mouse) Antibody

SKU: orb19453

Description

Goat polyclonal antibody to Cyld

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-1.5 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 125kDa band observed in mouse and rat brain lysates (calculated MW of 107kDa according to mouse NP_775545.1). Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Cyld (mouse)
• Molecular Weight: 107
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti CYLD antibody, anti cylindromatosis (turban tumor syndrome) antibody, anti 2010013M14Rik antibody, anti 2900009M21Rik antibody, anti C130039D01Rik antibody, anti CDMT antibody, anti CYLD1 antibody, anti EAC antibody, anti mKIAA0849 antibody, anti ubiquitin carboxyl-terminal hydrolase CYLD antibody

orb19453 (0.5ug/ml) staining of rat brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Mouse Brain using Cyld antibody

Quick Database Links

Gene Symbol

Cyld (mouse)

References

Yang, Xifeng et al. Downregulation of miR‑181b inhibits human colon cancer cell proliferation by targeting CYLD and inhibiting the NF‑κB signaling pathway Int J Mol Med, 46, 1755-1764 (2020)

PubMed: 32901872