CYK18 Antibody (C-term)

SKU: orb2649263

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF - 1:200, WB - 1:2000, IHC-P - 1:1000, FC - 1:50
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB18746
• Immunogen: This CYK18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from the C-terminal region of human CYK18. Antigen Region: 401-430 aa.
• Target: KRT18
• Molecular Weight: 48058 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18, CYK18

CYK18 Antibody (C-term) western blot analysis in WiDr, MDA-MB231, Hela, ZR-75-1, K562, NCI-H460, NCI-H292, HepG2 cell line lysates (35 ug/lane). This demonstrates the CYK18 antibody detected the CYK18 protein (arrow).

CYK18 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of CYK18 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

CYK18 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CYK18 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

CYK18 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CYK18 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

KRT18

UniProt

P05783

RefSeq (Protein):NP_954657.1, NP_000215.1