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CYK18 Antibody (C-term)
Description
Research Area
Images & Validation
−| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:2000 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 48058 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−CYK18 Antibody (C-term) [orb1929317]
FC, IF, IHC-P, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μlCytokeratin 18 Mouse Monoclonal Antibody [orb1474070]
WB
Human
Mouse
Monoclonal
Unconjugated
200 μl, 100 μl, 50 μl, 30 μl

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CYK18 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CYK18 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

CYK18 Antibody (C-term) western blot analysis in WiDr, MDA-MB231, Hela, ZR-75-1, K562, NCI-H460, NCI-H292, HepG2 cell line lysates (35 ug/lane). This demonstrates the CYK18 antibody detected the CYK18 protein (arrow).

CYK18 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of CYK18 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

CYK18 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CYK18 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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CYK18 Antibody (C-term) (orb2649263)
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