CYK18 Antibody (C-term)

• Catalog Number: orb1787843
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Target: This CYK18 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the 400-430 region of human CYK18.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG3
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: 400-430 aa
• UniProt ID: P05783
• MW: 48058 Da
• Tested applications: IF, WB
• Dilution range: IF: 1:25, WB: 1:2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Keratin, type I cytoskeletal 18, Cell proliferatio
• Note: For research use only
• NCBI: NP_954657.1, NP_000215.1

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoskeleton staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

All lanes: Anti-CYK18 Antibody (C-term) at1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: human liver lysate. Lane 5: mouse lung lysate. Lane 6: NIH/3T3 whole cell lysate. Lane 7: Rat liver lysate. Lane 8: C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.