CYK18 Antibody (C-term)

SKU: orb1787843

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Infectious Disease & Virology, Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF: 1:25, WB: 1:2000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3
• Immunogen: This CYK18 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the 400-430 region of human CYK18. Antigen Region: 400-430 aa.
• Target: KRT18
• Molecular Weight: 48058 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18, CYK18

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoskeleton staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

All lanes: Anti-CYK18 Antibody (C-term) at1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: human liver lysate. Lane 5: mouse lung lysate. Lane 6: NIH/3T3 whole cell lysate. Lane 7: Rat liver lysate. Lane 8: C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

KRT18

UniProt

P05783

RefSeq (Protein):NP_000215.1, NP_954657.1