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Cyclin D1

Catalog Number: orb1294339

DispatchUsually dispatched within 5-10 working days
$ 260.00
Catalog Numberorb1294339
CategoryAntibodies
DescriptionRabbit polyclonal antibody to Cyclin D1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit-IgG
ImmunogenSynthetic peptide / encompassing a sequence within the C-terminus region.
Dilution rangeWestern Blot 1:500-1:1000 Immunofluorescence 1:100-1:200 Immunohistochemistry (Paraffin) 1:100–1:200
ConjugationUnconjugated
TargetCyclin D1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NoteFor research use only
Expiration Date12 months from date of receipt.
Cyclin D1

Anti-Cyclin D1 antibody at 1/1000 dilution, Lysates at 30 μg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294339 overnight at 4°C.

Cyclin D1

Anti-Cyclin D1 antibody at 1/1000 dilution, Lysates at 30 μg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294339 overnight at 4°C.

Cyclin D1

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cyclin D1 with orb1294339 at 1/200.

Cyclin D1

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cyclin D1 with orb1294339 at 1/200.

Cyclin D1

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294339 Anti-Cyclin D1 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

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