Cyclin D1 Antibody (S90)

SKU: orb1931553

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:2000, IHC-P - 1:25
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB8066
• Immunogen: This Cyclin D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from human Cyclin D1. Antigen Region: 68-97 aa.
• Target: CCND1 {ECO:0000303|PubMed:8204893, ECO:0000312|HGNC:HGNC:1582}
• Molecular Weight: 33729 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1

Confocal immunofluorescent analysis of Cyclin D1 Antibody (S90) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Staining Cyclin D1 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Western blot analysis of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35 ug/lane). Cyclin D1 (arrow) was detected using the purified Pab.

All lanes: Anti-Cyclin D1 Antibody (S90) at 1:2000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Cyclin D1 Antibody (S90) at 1:2000 dilution. Lane 1: HT-1080 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

CCND1 {ECO:0000303|PubMed:8204893, ECO:0000312|HGNC:HGNC:1582}

UniProt

P24385

RefSeq (Protein):NP_444284.1